HPLC Grade Acetonitrile is prepared by a distillation process to remove the volatile impurities followed by filtration to achieve high purity solvents. These impurities found will contaminate the column and reduce the lifetime. HPLC Grade Acetonitrile is best suitable for liquid chromatography as it has a purity of at least 99.9 per cent. conforms to Acetonitrile for Chromatography and Acetonitrile R1 according to Reg.